cohort development

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“We have worked with inGenious Targeting Laboratory, Inc. on a variety of conventional, conditional and knockin projects, and we are very impressed with how flexible their service was to our needs and expectations. We look forward to our continued success in generating different mouse models with iTL.”– Iannis Aifantis, PhD NYU School of Medicine

cohort development

Genotyping Services: Available for existing and new custom mouse models designed by ingenious. Learn more and submit an order form.

Our Post Project Services Include

  • Breeding small or large cohorts of heterozygous or homozygous mice.
  • Breeding with any Cre lines (or tTA, tTS, CreERT etc.) with your new customized mice.
  • Genotyping of all offspring with established protocols developed by ingenious.
  • Timed deliveries at your request between your control and experimental groups.
  • Alleviate the need to justify animal use protocol numbers.
  • A free online tool to generate an estimated mating plan – ingenious Breeding Planner.
download the ingenious breeding planner

Tissue Harvest: Contact us to learn more.

Additional Services Include

Cryopreservation

Protect your mouse model and your research investment! We offer sperm cryopreservation and embryo cryopreservation services, various storage plans for cryopreserved sperm and embryos, and cryorecovery service to rederive your mouse line.

Sperm Cryopreservation

The service includes sperm collection and cryopreservation from two male mice, including motility test of thawed sperm. Service also includes in vitro fertilization and culturing of embryos to the blastocyst stage to confirm fertility of cryopreserved sperm. 1 year storage is included!

Embryo Cryopreservation

This service includes sperm collection from two male mice, and subsequent in vitro fertilization of oocytes from superovulated wildtype females. Embryos will be cryopreserved. 1 year storage included!

Note: Embryos will be a mix of wildtype and heterozygous genotypes when heterozygous males are used (the resulting pups must be genotyped), or all embryos will be heterozygous when homozygous males are used.

Once you receive your mouse model from ingenious, your ultimate goal will be to generate a cohort of mice of a certain age and sex, which you can then utilize for your experiments. You may continue to need more colonies as your experiments evolve. Depending on the model we have generated for you, this may require multiple mating steps, genotyping for various genetic elements, and calculated scaling up to obtain the specific number of mice with the appropriate sex, background and genotype.

We know from talking with many of our clients that traditional in-house breeding often becomes an unnecessarily expensive and time consuming process, which can even lead to the loss of the mouse line. ingenious offers downstream services to make this important endeavor seamless.

By outsourcing the generation of mouse cohorts to ingenious, you will benefit from expert staff that will work with you to determine the most efficient breeding plan to meet your research goals. Utilizing our proprietary breeding software allows us to develop plans of action based on time lines and costs that best fit your lab.

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