A standard conditional knockout is a versatile tool for studying your gene of interest – there are as many possible patterns of knockout as there are Cre lines. With hundreds of our conditional knockout models published, our experts will evaluate the structure of your gene and help to determine the best design for your research goals and aspirations.
Start your purchase!“We are actively engaged in the production of a number of conditional mouse models with iTL. This collaboration has been simple on my end, just providing the gene accession numbers of each gene, and iTL recommending the strategies for each gene. The full range of mouse knockout services matches my needs well. I find iTL’s service uniquely useful for my situation of needing different models for my research in a quick and efficient manner.”– Mehboob Hussain, MD University of Michigan Health
The schematic below illustrates the targeting strategy of a conditional knockout mouse model in which exon 2 was flanked with loxP sites, and the Neo cassette was introduced into the intron downstream of exon 2. Upon FLP and Cre recombination, exon 2 is eliminated, and a loxP/FRT footprint remains.
The deletion of exon 2 results in a frame shift and early stop codons. The mRNA produced from this disrupted gene is targeted by the nonsense-mediated decay pathway, generally resulting in no protein being produced.
A specific target sequence within a gene of interest is flanked by loxP sites to facilitate Cre recombinase-mediated excision, for tissue specific or temporally controlled gene inactivation. The following general considerations apply for conditional knockout mouse models and are evaluated by our experts for each conditional knockout model generated at ingenious.
The model you design is the model we deliver.
Assurance of your mouse model, not just your money back.
Our proprietary technologies save time and money.
Phone: +1 (631) 468-8530
