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Partial Services


Offering vector creation and gene targeted ES cell lines.

Take advantage of our expertise and the services offered by your core facility. Together we design a custom DNA targeting vector for your specific needs. Our scientists handle the complex cloning and verification steps, then you work with your core facility to produce your custom mouse model.


Donor/Repair Vectors for CRISPR/Cas9 Gene Editing

CRISPR/Cas9 technology has brought rapid progress in mammalian genome editing by increasing the ease and frequency of targeted events. But a limitation is the size of single-stranded oligos that can be readily produced, making genetic modifications spanning larger distances challenging. This rules out efficient production of many popular model types such as conditional alleles and gene knockins. Larger vectors that introduce such modifications require larger homology arms (3kb or more) for efficient recombination, and creating such a vector can be challenging and time-consuming.

Let our experts create the donor vector that you need for your CRISPR project in record time and without the cloning headaches.

Some examples:

Any size cDNA insertion vectors
loxP (conditional KO) vectors with recombinase sites spaced beyond 1 Kb apart
Reporter gene knockin vectors
Combinations of any of the above
Or contact us to discuss your insertion need

Gene Targeted ES Cell Lines

ingenious delivers custom gene targeted ES cells. We design and build the targeting vector, electroporate it into our robust ES cells, and subsequently screen the ES cell clones by PCR and Southern Blotting. The targeted clones can be microinjected into blastocysts at your core facility to generate chimeras and subsequently F1 mice.

We guarantee the generation of positively targeted ES cell clones if all work is performed at our facility. This includes all troubleshooting, if necessary, at no additional cost.

Utilizing our FLP ES cell lines alleviates the process for deleting the Neomycin selection cassette, saving you at least 3 months of time, and valuable funding.

Starting with a Targeting Construct Provided by a Third Party

Starting with KOMP/EUCOMM Targeting Constructs or Targeted ES Cell Clones

Working with a Targeting Vector from the Consortium

Working with Targeted ES Cell Clones from the Consortium

After You Receive Your Mice

Resources

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