At ingenious targeting laboratory, we utilize precision gene targeting to generate your transgenic mouse model. Our proprietary technologies allow for shorter production timelines and reduced costs when compared to other targeting methods, without compromising performance and quality. We efficiently introduce your overexpression construct into the genome in a location where it will not interfere with other genes or be affected by the surrounding genetic environment, for more predictable results.
Various options are available for the customized design of your transgenic mouse model, depending on your unique needs, including knockin to specific genes, safe-harbor transgenic knockin, conditional overexpression, and drug-inducible systems. Our models are versatile tools for studying human disease, drug testing, and gene function analysis.
“iTL has done a tremendous job assisting us with our projects. Not only have they provided successful mouse lines, but their project management has always been on top of things. Communication was excellent, and at all times I felt I could trust the scientists at iTL with my work.”– Thomas G.H. Diekwisch, DMD, PhD (sc), PhD (phil.) Texas A&M University College of Dentistry
ingenious has taken the complicated process of generating tissue-specific inducible transgene expression systems and simplified it. By designing a targeting vector that contains a floxed stop cassette and all of the necessary Tet-On control components, we enable tissue-specificity and inducibility without the need for transactivator or silencer mouse lines. Simply mate the targeted transgenic line with a tissue-specific Cre recombinase mouse line of choice and introduce doxycycline for inducible/reversible expression of your transgene in your tissue of interest.
The design works by incorporating a reverse tetracycline-controlled transactivator (rtTA) component, a TRE3G (tetO) promoter, and a floxed Neomycin-Stop cassette into the “safe harbor” Rosa26 locus. The Neomycin-Stop cassette, inserted downstream of the CAG promoter, prevents rtTA expression, which is one of the key components required for transgene expression from the TRE3G promoter.
The model you design is the model we deliver.
Assurance of your mouse model, not just your money back.
Our proprietary technologies save time and money.
Phone: +1 (631) 468-8530
