Inducible Rosa26-Express™ Targeting

Improved inducible and tissue-specific transgene control.

Inducible transgene expression systems represent a key methodology for studying gene expression and function. Direct, inducible control over transgene expression allows for developmental and time point studies in vivo. Developing mouse models with inducible expression systems, however, can be quite complicated and costly, and require mating with transcriptional activator or silencer mouse lines that might not already exist, when tissue-specificity is desired.

Introducing ingenious’ Inducible Rosa26-Express™ Targeting

ingenious has taken the complicated process of generating tissue-specific inducible transgene expression systems and simplified it. By designing a targeting vector that contains a floxed stop cassette and all of the necessary Tet-On control components, we enable tissue-specificity and inducibility without the need for transactivator or silencer mouse lines. Simply mate the targeted transgenic line with a tissue-specific Cre recombinase mouse line of choice and introduce doxycycline for inducible/reversible expression of your transgene in your tissue of interest.

Inducible Rosa26-Express™ Targeting Benefits:

• Improve Inducible and Tissue-Specific Transgene Control from the Safe-Harbor Rosa26 Locus: ingenious’ Inducible Rosa26-Express™ system allows for easier inducible and tissue-specific control of transgenes.
• Reduced Time and Cost: Our Inducible Rosa26-Express™ is combined with our Rapid-Rosa26™ Targeting technology to reduce the time and cost required to generate your mouse model.
• Tissue Specificity via Cre Recombinase: Tissue specificity is determined by Cre recombinase expression. With a vast array of Cre expressing mouse lines readily available, finding the expression profile you need is easy and reliable.
• Control Transgene Expression: Doxycycline provides inducible control over your transgene and allows for expression at specific ages, or for defined durations to meet experimental requirement.
• Avoid Undesirable Embryonic or Developmental Phenotypes: Embryonic and/or developmental phenotypes can be a risk when introducing transgenes, especially if the transgenes are novel or not well characterized. Bypass these issues by preventing expression during early developmental stages.

How ingenious’ Inducible Rosa26-Express™ Targeting System Works

The design works by incorporating a reverse tetracycline-controlled transactivator (rtTA) component, a TRE3G (tetO) promoter, and a floxed Neomycin-Stop cassette into the “safe harbor” Rosa26 locus. The Neomycin-Stop cassette, inserted downstream of the CAG promoter, prevents rtTA expression, which is one of the key components required for transgene expression from the TRE3G promoter.