TruHumanization™
Traditional approaches to humanizing genes in the mouse all lack one or more elements of gene regulation. In addition to upstream promoters and untranslated regions in mRNA, expression can be affected by promoters located in introns, by splicing, and by the gene’s chromosomal location. Only full gene replacement recapitulates all these features. Contact us today to discuss a new gene replacement model.
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Work with ingenious for your custom knockout mouse model.
Certainty
The model you design is the model we deliver.
Guaranteed Delivery
Assurance of your mouse model, not just your money back.
Value
Our proprietary technologies save time and money.
purchaseAdvantages of Fully Humanized Mouse Models
Let ingenious create the ultimate model for you by fully humanizing your gene of interest. Using TruHumanization™ technology up to 200kb can be replaced in a single step, including coding and noncoding sequence. Your new model will be a true gene replacement: the mouse sequence is eliminated and replaced with human genomic sequence.
A humanized gene can also include a reporter, or be made into a conditional allele for increased control over expression, and can incorporate known disease-causing mutations. Fully humanized mouse models lead to more faithful gene regulation and processing as well as the potential to perform functional and regulatory studies on multiple transcripts, providing you with a more flexible mouse model to study for years to come.
download our humanization white paperClient Publications Using TruHumanization™
Saito T, Mihira N, Matsuba Y, Sasaguri H, Hashimoto S, Narasimhan S, Zhang B, Murayama S, Higuchi M, Lee VMY, Trojanowski JQ, Saido TC. 2019. Humanization of the entire murine Mapt gene provides a murine model of pathological human tau propagation. J Biol Chem294(34): 12754-12765.
