CRISPR Mouse Models
At the speed of CRISPR. Generate models quickly and efficiently.
Germline Transmission Guaranteed
Through our collaboration with Shanghai Model Organisms Center, Inc. (SMOC), ingenious targeting laboratory is proud to offer select, high-quality, custom CRISPR mouse models with germline transmission guaranteed.
Fill out our quote form to find out how we can apply the power of CRISPR/Cas9 genome editing for your next project, whether it is a guide RNA-directed gene inactivation or introduction of a single point mutation.
Germline transmission guarantee available for these mouse models:
Conventional Knockout
Cas9 generates targeted cuts in your gene of interest which are repaired by an error-prone DNA repair pathway, i.e., non-homologous end joining (NHEJ). Mutations introduced by this process disrupt the gene either by nucleotide insertions or deletions (INDELs) to create a knockout allele.
Constitutive Point Mutation
Single nucleotide changes are introduced at a target site by combining CRISPR/Cas9 with a modified DNA template (e.g., an oligo) in order to support a repair pathway which utilizes homology-directed repair (HDR). Commonly this strategy applies to the modeling of disease-causing mutations or the humanization of critical amino acids.
The 3 Step ingenious-SMOC Process for Generating Your Custom CRISPR Mouse Model
Scientific experts at ingenious and SMOC will work with you to design and customize your genetically engineered constitutive KO or point mutation KI mouse model, to your specifications.
1.
1st Step: Strategy and Design
Identification of target site, guides, and oligonucleotides, as well as predicted off-target sites. Preparation of sgRNA and Cas9 mRNA.
2.
2nd Step: Production of F0 Founder Mice
Injection of CRISPR/Cas9 gene editing material into fertilized embryos to generate F0 mutated candidate animals resulting from embryo transfer into pseudopregnant mice.
3.
3rd Step: Breeding of Founders to Obtain F1 Mice
The new founders are bred with wildtype mice of matching strain background, and their offspring are genotyped to identify and select F1 mice bearing the intended knockout or knockin allele originating from the genetically heterogeneous (i.e., mosaic) F0 founder.
Faster Founders with CRISPR
CRISPR/Cas9 enables the creation of founder mice for your new line in less time and at a lower cost compared to cell-based approaches. Previously to make a knockout line the modification had to be made and verified in ES cells, which can take three months or more, then potential chimeric founders had to be generated from those cells. CRISPR eliminates this initial step and creates genetic changes directly in embryos, so that screening for potential founders can begin months earlier in relation to ES cell-derived animals. We are looking forward to working with you to create an indispensable mouse line in support of your research.
Notable Publications by SMOC Clients
1) Zhao H, Huang X, Liu Z, Pu W, Lv Z, He L, Li Y, Zhou Q, Lui KO, Zhou B. 2021. Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas. Nat Metab 3(3): 352-365.
2) Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H. 2018. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci 21(3): 440-446.
3) Huai C, Jia C, Sun R, Xu P, Min T, Wang Q, Zheng C, Chen H, Lu D. 2017. CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice. Hum Genet 136(7): 875-883.
4) Xie C, Zhang YP, Song L, Luo J, Qi W, Hu J, Lu D, Yang Z, Zhang J, Xiao J, Zhou B, Du JL, Jing N, Liu Y, Wang Y, Li BL, Song BL, Yan Y. 2016. Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Cell Res 26(10): 1099-1111.
