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cDNA Knockin

Precisely express your sequence of interest in an animal model.

Expressing a specific sequence in place of a target gene, or in addition to that gene, is a powerful and versatile strategy. A cDNA mouse knockin model allows expression of a new sequence using most regulatory features of an endogenous gene: the upstream promoter, the 5’ UTR and the chromosomal environment. This strategy allows expression of human sequence in place of mouse, tissue-specific expression of Cre or a reporter gene, and more. We can also make your cDNA expression conditional by including loxP sites in the design. Contact us to discuss available options.

Express cDNA in Place of Target Gene

Co-Express cDNA Along with Target Gene

Conditional + Reversible Design

cDNA Knockin to Replace Expression of Target Gene

cDNA Knockin for Co-Expression with Target Gene

Conditional+Reversible Design

Client Publications Using cDNA Knockin

Frequently Asked Questions

What is cDNA and how is it used?

DNA is composed of both coding and non-coding sequences, whereas cDNA (or copy DNA) contains only coding sequences.  cDNA is used as a tool in gene cloning and other research experiments.

What change is made to DNA in knock-in mice?

Gene knockin technology alters the genetic locus of interest via a one-for-one substitution of DNA sequence by the addition of sequence information that is not found on the genetic locus.

What happens to the DNA in Knockin mice?

Knockin mice may have a new genetic sequence added that is turned on in all cells, or only certain cells, or in response to mice receiving a specific drug. The knockin gene might cause a disease in the mice, or correct a problem caused by a different mutation, or mark certain cells with a fluorescent protein label.

RELATED: Difference Between Knock In And Knockout

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