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Buy Transgenic Mice from ingenious


Are you at a point in your research where you need a new custom mouse model? Are you unable to find an off-the-shelf model that is suited to your research? A better mouse model can be the next step for your research no matter what you study. If you’re wondering where to buy transgenic mice, look no further than ingenious targeting laboratory. We understand that purchasing an animal model may seem like a complicated process, which is why we’ve put together this guide to simplify it for you.

Frequently asked questions about transgenic mice

1) What is a transgenic mouse model?

A transgenic mouse is a type of mouse model with an extra genetic sequence inserted into its genome. This sequence, also known as a transgene, varies from model to model as each transgenic mouse model is specifically designed with certain experiments in mind. Many off-the-shelf transgenic models are readily available but if you can’t find a suitable line it may be necessary to generate a custom transgenic mouse. This is crucial to keep in mind when you’re looking to buy transgenic mice for your next research project. A simple genetic design for a transgenic construct is shown here:

Knocking in a constitutive promoter and cDNA will give strong expression across all tissues. Different knockins can be used to achieve tissue-specific expression or drug-inducible expression.

Transgenic mice became widely available in the 1980s including the famous oncomouse published in 1984 for cancer studies. It was this model, as well as other transgenic mice that were generated in the next few years, that helped the field of cancer research grow into what we know it as today. That’s because oncogene transgenic mice allowed scientists to observe the development of cancer in certain organs that could not be replicated in tissue cultures. Now, it is possible to buy transgenic mice that model many types of organ-specific cancers and the particular characteristics that come with each one. [1]

Why Use Transgenic Mouse Models?

2) Where can I buy a mouse model?

ingenious targeting laboratory offers custom mouse models that are tailored to our clients’ specifications and research needs. We established ourselves as one of the very first mouse gene targeting companies in 1998. Since then, our models have been featured in over 600 client publications including studies published in ScienceNature, and Cell. Our clients choose to buy transgenic mice and work with us again and again because of the quality of our work and the strong relationships we build with them.

Once you decide to partner with us, our scientific consultants will guide you through the process of buying transgenic mice. We begin with a quote consultation that will help us determine what type of model you’d like to generate and what strategies are available. This is a good first step before you order a mouse model online.

3) How much do transgenic mice cost?

For pricing information, please download ingenious’ Pricing Guide or contact us for a custom quote. What sets us apart from other companies is that when you buy transgenic mice from ingenious, you are guaranteed to get your model, not just your money back. Because we’re backed by over 20 years in business we are proficient in working with both classic and cutting-edge technologies.

Our team of experts is standing by to help get your new project off the ground quickly. We will be there with you every step of the way to make sure your mice are accurately targeted and arrive safely at your facility. You can also elect for our other available mouse generation services, such as cohort generation, colony management consultation, cryopreservation, genotyping, and more to help you get the most out of your new custom mouse line.

4) Why should I use targeted transgenics?

When buying transgenic mice, it’s important to consider whether you may need targeted insertion over random insertion. With targeted transgenics you can expect more predictable and consistent results. That’s because your construct is inserted into a specific genomic location, such as a safe harbor locus like Rosa26. While ingenious specializes in working with the Rosa26 locus, other safe harbor locus options are available. Some examples of models that can be generated by targeting the Rosa26 locus are constitutive expression, tissue-specific expression, and conditional expression. Targeted transgenics are by no means limited to the use of safe harbor loci however. The advantage of this powerful method is that a sequence can be inserted almost anywhere, for example to modify the expression pattern or protein product of a gene of interest.

Random insertion, on the other hand, results in your transgene being inserted somewhere in the genome with no control over its location. While this may be suitable for some studies, you may find that you require your construct to be placed in a specific location in order to accurately study your gene of interest. Otherwise, your research may be left up to chance. Today the methods of the 1980s are still used to generate random insertion transgenic lines because founder mice can be produced quickly and at low cost. These advantages are greatly reduced when you consider that each potential founder has the transgene integrated at a different site. Offspring from multiple founders must be carefully screened to find mice where the transgene functions as planned.

For more information, please read our blog post 4 Reasons To Use Targeted Transgenic Mice Over Random Insertion.

5) How do you make transgenic mice?

Transgenic Knockin to Your Gene of Interest

With a targeted cDNA knockin it’s possible to alter a gene in the mouse genome and make it express a reporter gene or other alternate cDNA sequence, for example the sequence of a human gene. The sequence of Cre recombinase can be inserted using this method to create a new tissue-specific expression line. This is important to consider the next time you’re looking to buy transgenic mice.

Targeted knockins often inactivate the mouse gene and result in the transgene being expressed in its place. However, the knockin strategy is flexible and it’s possible to co-express the cDNA along with your target gene. This makes it possible, for example, to generate a line that expresses Cre along with the targeted gene.

Safe-Harbor Transgenic Knockin (Rosa26)

ingenious targeting laboratory developed proprietary technology for generating transgenic models using the Rosa26 locus: Rapid-Rosa26™ Targeting. The advantage of using a safe harbor locus is that your overexpression construct can be introduced into a location that won’t interfere with surrounding genes, thus isolating it. This results in targeted expression that is more predictable.

Rapid-Rosa26™ Targeting

With Rapid-Rosa26™ targeting, when you buy transgenic mice, you can expect shorter timelines and a reliable, high-quality model. One concern researchers may have about targeted insertion is that it may take longer to generate, and at a higher cost. However, ingenious’ Rapid-Rosa26™ technology allows us to generate a custom transgenic model with accurate targeting on shorter timelines, enabled by vectors that have been created with thoroughly validated strategies. An example of a standard strategy is shown here, for conditional expression of a specific cDNA. Tissue-specific expression is achieved by crossing the Rosa26 transgenic line to one of the hundreds of available Cre-expressing lines.

6) How can I buy transgenic mice from ingenious?

If you’re ready to buy transgenic mice for your next research project, get in touch with ingenious targeting laboratory. Fill out our quote form and one of our scientific experts will provide you with a custom quote within 1 business day.


References

1) Hanahan D, Wagner EF, Palmiter RD. 2007. The origins of oncomice: a history of the first transgenic mice genetically engineered to develop cancerGenes & Development 21: 2258-2270.


Notable Client Publications

  1. Mlynarczyk C, Teater M, Pae J, Chin CR, Wang L, Arulraj T, Barisic D, Papin A, Hoehn KB, Kots E, Ersching J, Bandyopadhyay A, Barin E, Poh HX, Evans CM, Chadburn A, Chen Z, Shen H, Isles HM, Pelzer B, Tsialta I, Doane AS, Geng H, Rehman MH, Melnick J, Morgan W, Nguyen DTT, Elemento O, Kharas MG, Jaffrey SR, Scott DW, Khelashvili G, Meyer-Hermann M, Victora GD, Melnick A. 2023. BTG1 mutation yields supercompetitive B cells primed for malignant transformation. Science 379(6629): eabj7412.
  1. Gleixner AM, Verdone BM, Otte CG, Anderson EN, Ramesh N, Shapiro OR, Gale JR, Mauna JC, Mann JR, Copley KE, Daley EL, Ortega JA, Cicardi ME, Kiskinis E, Kofler J, Pandey UB, Trotti D, Donnelly CJ. 2022. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13(1): 3380.
  1. Kasatkina LA, Ma C, Matlashov ME, Vu T, Li M, Kaberniuk AA, Yao J, Verkhusha VV. 2022. Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model. Nat Commun 13(1): 2813.

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